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Pediatric pharmacokinetics and long-term safety require clarification, particularly for polyphenols and carotenoids with intrinsically low oral bioavailability and potential interactions with conventional medications like antipsychotics or stimulants (Hendouei et al., 2020

Alternative approaches with the inversed configuration have been developed in which gRNAs with different sequences are used as barcodes, and a CRISPRa reporter containing a target sequence is introduced for barcode-specific fluorescent reporter activation 30,31