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Other microbial metabolites, such as polysaccharide A and retinoic acid produced by commensal Clostridia , Faecalibacterium prausnitzii , and Bacteroides fragilis , induce the accumulation of regulatory T (Treg) cells, which suppress inflammation (Forbes et al., 2016)

The absorbance at 570 nm was measured in a plate reader

First, 0.4 mg/ml TGR protein was mixed with 5 mM of inhibitor 9 in buffer solution containing 0.15% DMSO and incubated 30 min at room temperature (20 C)

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Acteoside was also observed to down-regulate pro-caspase 3, decrease expression of Bax, and increase expression of BCl2 compared to controls [30]