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Amplex-type assays report pooled H 2 O 2 and therefore integrate multiple mitochondrial and extra-mitochondrial sites, so accurate source attribution requires site-specific probes (including genetically encoded reporters targeted to matrix, IMS, or outer membrane) and carefully controlled substrate and inhibitor protocols that isolate individual electron-transfer nodes (Waldeck-Weiermair et al

FAD is important for producing energy both from carbohydrates and through fatty-acid oxidation in the mitochondria.[ref] FAD is also involved in detoxification as a cofactor for some CYP450 enzymes, as well as for glutathione recycling

Since the Raman spectra are dominated by the amide bands of the peptide backbone, similar to ORD and CD, it can be used for the analysis of protein secondary structure by finding correlations between the positions of the amide I and amide III vibrations and fraction of each secondary structural element present in globular proteins determined from X-ray crystal structures (Williams, 1986

Glutathione may also prevent heart problems and Alzheimers Disease, among other health conditions

Paige LA, et al