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Below is the standard duration for each skin colour: Medium brown skin: 1-3 months Dark brown skin: 3-6 months Very dark skin: 6-12 months Black skin: 2 years or more

Samples were diluted in 1x NuPAGE LDS Sample Buffer with a final of 80 mM DTT (ThermoFisher Scientific), heated at 95C for 5 minutes, and processed further for western blotting

doi: 10.1530/jrf.0.1200257 96

Results were categorized thematically according to the main headings in the article, with preclinical and clinical findings separated for clarity

The trade-off: Injections deliver the molecule most efficiently, but they dramatically raise the "risk floor" due to issues like sterility and systemic allergic responses