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Rearranging (12) and solving for M gives Equation 14: [M] = [Mo] -2[D] Substituting (14) into the Kd expression (1) gives Kd = (Mo-2D)(Mo-2D)/D where can be rearranged into quadratic form: Equation 15: 4D2 - (4Mo+Kd)D + (Mo)2 = 0 which is of the form y = ax2+bx+c