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However, in the absence of evidence for cysteinylation of KEAP1 in cells, we cannot completely exclude the possibility that Cys signaling is mediated indirectly

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For PNGase H+ application, exchanging with distilled water was omitted, and slides were immediately dried in a desiccator after antigen retrieval to ensure acidic conditions necessary for optimal PNGaseH+ activity

Control rats were not given the GEY/RES