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These percentages are almost identical to the percentages for the second-order rate constants of the same mutants with GSSCys as a substrate 14 , indicating that GSH also occupies the 1st glutathione-interaction site of PfGrx for the reduction of roGFP2 WT (S 2 )

Instead of DTT and N-ethylmaleimide 0.3 ml H 2 O was added to measure in a similar reaction mixture

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These changes contribute to neuroinflammation, bloodbrain barrier (BBB) dysfunction, microglial activation, and neurotransmitter imbalances