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Proteins were separated by 10% SDS-PAGE and transferred to a PVDF membrane (Millipore)

T2DM is characterized by a proinflammatory state wherein there is an overproduction of cytokines which can damage the vasculature, interrupt hemodynamics, and increase the risk for infection ( M

On the other hand, a positively charged Arg at this position, which is present in the APOE4 protein, forms a salt bridge with Glu109 and this salt bridge causes exposure of the positively charged Arg61 on the protein surface

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