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In particular, an experiment based on the -glucosidase-catalyzed hydrolysis of standard vicine to divicine, described in detail in Section S2 of the Supplementary Information, provided an approach to distinguish divicine eventually generated during faba bean flour fermentation from that arising from the gas phase spontaneous fragmentation of vicine during ESI-MS analyses

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Cells were next permeabilized with 0.5% Triton X-100 in PBS for 10 min, washed once with PBS, and blocked for 30 min in 2% BSA in PBS

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