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Samples were then desalted again, and fractionated to 12 fractions using a 57-minute linear gradient from 3% to 32% acetonitrile (ACN) in 10 mM ammonium bicarbonate pH 8.0, at a flow rate of 0.25 mL/min, with an Agilent 1100 HPLC

JanickaMKot-WasikAKotJNamienikJ

The study showed an absolute 5.5% smaller myocardial infarct size in NAC-treated patients compared with placebo-treated patients (16.5% versus 11.0% size reduction, p = 0.02)

doi: 10.1002/cpt.1548 193

Hence, we next investigated whether we could use our human assembloid model containing portal fibroblasts to recapitulate aspects of human liver disease in vitro, specifically biliary fibrosis