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The remaining 23 mL of each culture was quickly filtered under vacuum (Air Cadet vacuum/pressure station, Thermo), immediately washed with cold saline solution (9.5 g L 1 NaCl at 12 C) and quenched in cold methanol/water (1:1 v/v) at 30 C as described by Smart et al

Zhang Y, Lv YL, Si YN, Zhou J, Qian Y, Bao HG

& Gespach, C

A contaminated or degraded solution will typically show at least one of: cloudiness or turbidity, visible particles or flakes, color change from clear to yellow or brown, or an unusual odor

The commenters stated that all three procedures need to be reassigned because the CY 2024 claims data for CPT code 0600T shows a geometric mean cost of $13,068, which is higher than the proposed APC 5362 geometric mean cost of $11,137