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periodic breaks are recommended as a precaution [6]

94 Previous studies have shown that the formation of cell-filled ALG microspheres from an aqueous solution of ALG gel precursor and CaCl 2 using the LIFT technique and the formation of a thick gel film during droplet descent significantly increased fibroblast activity, but at the same time, this impeded the diffusion of nutrients and oxygen through the medium, resulting in a decrease in cell viability, and that cell viability decreased with increasing laser fluence or ALG concentration

This suggests that a primary difference between the GF and SM protocol in terms of their individual effect on iPSCs during differentiation is the extent of metabolic rewiring and maturation in the HLCs

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For researchers working with these compounds, investing in a dedicated laboratory-grade refrigerator with temperature logging is non-negotiable