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If long-term storage of a reconstituted solution is required, researchers typically aliquot the solution into single-use vials, snap-freeze at -80 C, and thaw only once before use

For comparisons among three or more groups, one-way or two-way ANOVA with Bonferroni post hoc correction was applied

Hydroxocobalamin offers superior absorption, longer retention, and greater efficacy versus cyanocobalamin and methylcobalamin, making it the preferred choice for severe deficiency and for those needing frequent injections

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