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Fukugawa NK, Ajami AM, Young VR

As OxS plays an important role in PSC activation and pancreatic fibrosis, we hypothesized that high glucose could stimulate pancreatic cells to produce excess ROS and activate PSCs, that induce pancreatic fibrosis and ultimately lead to -cell failure

For the analysis of the expression of endogenous Munc13-4 and STX7 in HEK293T cells, 10 g of protein was resolved by gel electrophoresis carried out using 412% gradient or 12% Bolt BisTris Plus gels (Life Technologies)

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