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second, the VTA models may approximate the real volume of tissue activated but not fully represent reality, and the estimation of the VTA did not involve the impact of stimulating frequency

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The sample was analyzed via UV-Vis, then the temperature was raised of 4 K at a time stirring the solution for 1 min before leaving the system to equilibrate for another 15 min and repeating the acquisition every 5 min until two consecutive spectra are superimposable between 288 and 298 K, then 2 K per step until 327 K