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Animals fed a Cu-deficient diet also develop emphysema, as Cu deficiency prevents LOX activation (Zajac, 2021)

The protein levels in the homogenate were assayed using protein assay reagent (Bio-Rad, Hercules, CA) to normalize the amount of lipid extracted

Arch Dis Child Fetal Neonatal Ed 88(4):F308F311 Xiang-wei H (2013) Influence of naloxone on respiratory mechanics, pulmonary function parameters and oxygen metabolism in patients with chronic obstructive pulmonary disease and respiratory failure [J]