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Access this article Similar content being viewed by others References YS Ilhan N Bulbuller C Kirkil R Ozercan D Seckin (2005) ArticleTitleThe effect of an angiotensin converting enzyme inhibitor of intestinal wound heaking J Surg Res 128 615 Occurrence Handle15869762 Occurrence Handle1:CAS:528:DC%2BD2MXpsVWks7k%3D P Sikiric S Seiwerth Z Grabarevic M Petek R Rucman B Turkovic et al

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Single-cell suspensions were then loaded onto microfluidic devices, and single-cell RNA sequencing (scRNA-seq) libraries were constructed according to the Singleron GEXSCOPE protocol via the GEXSCOPE Single-Cell RNA Library Kit (Singleron), which included cell disruption, mRNA trapping, cell labeling (with barcodes) and mRNA labeling (with unique molecular identifier (UMI)), reverse transcription of mRNA into complementary DNA (cDNA) and amplification, cDNA fragmentation, and finally cDNA fragment cloning