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Our team recommends it for research demanding maximal efficacy and minimal variability

One member abstained

Ascorbate measurement For neutrophil ascorbate measurement, 10 l of the extraction solvent (80% methanol containing 10 mM EDTA and 50 pmoles 13 C-ascorbate/200,000 cells as an internal standard) was added to 10 L neutrophil extract, vortexed for 10, centrifuged at 21,000 g for 15 min at 4 C, the supernatant was diluted using 30 L of 0.03% formic acid in water and analyzed using an AB Sciex 6500+ QTrap mass spectrometer coupled to a Shimadzu UHPLC system operating in negative mode to detect the transitions 175/115 (ascorbate) and 176/116 ( 13 C-ascorbate)

Most preservative-free sterile waters are unsuitable for repeated access once opened, as sterility cannot be maintained after the initial puncture

These resources include: A CISP Checklist Implementing Revised List (available now) Athlete and Athlete Support Personnel (ASP) Factsheet on Glucocorticoids (to be available in October) Medical Professionals Factsheet on Glucocorticoids (to be available in the coming weeks) Athlete and ASP Guide to the 2022 List (to be available from 1 January 2022) UPCOMING WEBINAR In order to further assist stakeholders in their understanding and implementation of the 2022 List, WADA will host a webinar in November 2021