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Epub 2019 Jan 28

The cells were passaged weekly in a 1 to 3 ratio, using PBS (Medicago, Uppsala, Sweden) for washing and 0.25% Trypsin-EDTA (Sigma-Aldrich, Steinheim, Germany) for detachment

Solvent A was added to the cell pellet, vortexed for 10 s, and allowed to sit on ice for 3 min

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