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qPCR with reverse transcription This was performed in total RNA samples, purified from primary cultures of astrocytes and neurons using the GenElute Mammalian Total RNA Miniprep Kit (Sigma), following the manufacturers protocol

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Vitamin B2 is provided in the active and bioavailable form of riboflavin-5-phosphate (R-5-P)

One wrong move, whether it is squirting water directly onto the lyophilized powder, shaking the vial to speed up dissolution, or choosing the wrong diluent, can degrade the peptide before you ever draw your first dose

Bioprocess and Biosystems Engineering, 32 (6), 845852