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The assay based on the reaction of GSH with 5,5-dithio-bis-2-nitrobenzoic acid (DTNB) in the presence of glutathione reductase, was performed according to the protocol 56 and measured with a TECAN plate reader at 412 nm

500 M H 2 O 2 for 10 min followed by 100 M GSH ethyl ester for 10 min

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In this guide, Dt

Glutathione is decisive in either limitingor preventing the harmful effects induced by free radicals,and canprotect the body from oxidising agents, such as pesticides, hydrogen sulphide, carbon monoxide, heavy metals (mercury, cadmium and chromium), cigarette smokeand many other substances we come in contact withdue to pollution of the atmosphere and of foodstuffs