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Alternatively, low-molecular weight crosslinker moieties may be used, especially when hydrophilic high-molecular weight backbone moieties are used for the reactive biodegradable hydrogel formation

Approximately 5 h post-seeding, the culture medium was replaced with neurobasal medium containing 2% B27 and 0.5 mM glutamate, and then maintained at 37 C in a 5% CO2 atmosphere