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H 2 O 2 -dependent signaling occurs with a lower intracellular concentration of approximately 1-100 nM, mediating reversible oxidation of the thiolate group to a sulfenic group (Cys-SOH) and covalent linkage of cysteine residues by disulfide bonds (Cys-S-S-Cys) altering its activity and localization ( 2 O 2 mediates irreversible nonspecific oxidation of proteins, resulting in a state referred to as oxidative stress ( ROS regulate cellular homeostasis and signaling events within a cellular environment, thus serving as primary modulators of cellular dysfunction and contributing to disease pathology

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