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doi: 10.1152/ajprenal.00472.2009

The TGR sample (1/50 volume cell culture) was applied to a 2.5 ml column, which was then washed with 50 ml of TE after which TGR was eluted in ten 1 ml fractions of 1 mM NADPH in TE

However, though some studies also reported some plasma biomarkers for brain oxidative damage by analyzing different kinds of samples including F4-Neuroprostanes and F2-Dihomo-Isoprostanes (278), biomarkers in peripheral body fluid samples are still insufficient to identify brain or peripheral oxidative stress at the moment

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