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The principle of this assay is the following: the general MAPK phosphorylation target motif (S/TP) is positioned C-terminal from a MAPK binding D-motif and this SENSOR construct is fused with the small fragment of the luciferase enzyme (NanoBiT small subunit, deep sea shrimp, Oplophorus glacilirostris , Promega)

The results are not comparable in terms of depth, duration, or visible impact on skin quality

only the amount of ligand reaching the receptor differs

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Step 5: Verify the Solution The reconstituted solution should be completely clear with no visible particles, cloudiness, or discoloration