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(2019) evaluated the effectiveness of administering vitamin D, DHA and both (VIDOM) on 117 children

In brief, 7 l of RNA was mixed on ice with 10 l 2 cDNA Synthesis master mix, random primers: oligo primers 2:1 (total 3 l), 2 l deoxynucleotide mix (1 mM each: dATP, dTTP, dCTP and dGTP), 0.5 l RNase inhibitor (40 U l 1 ), 0.5 l reverse transcriptase (20 U l 1 ) and 3 l nuclease-free water