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Understanding these possible contributors can help you feel less frustrated and more empowered to notice patterns

Separations on the LC-MS were performed on a CAPCELL CORE C18 (2.7 m, 2.1 50 mm, SHISEIDO Co., Ltd., Tokyo, Japan) using a linear gradient of 5100% solvent B over 8 min at a flow rate of 0.4 mL/min