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Prytzyk E, Dantas AP, Salomo K, Pereira AS, Bankova VS, De Castro SL, Neto FR

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The reaction was allowed to proceed for 30 s and was terminated by adding 80 L of a methanolic quenching solution, which consisted of 375 L of 100 mM Tris buffer (pH 9.5) with 0.2 mM EDTA and 625 L methanol