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The bacterial cells were grown at 20 C and after 4048 h, the cells were harvested by the centrifugation at 6500 g for 10 min, washed 23 times with phosphate-buffered saline (PBS) buffer (pH 7.2) and the cell pellet was re-suspended in ice cold 20 mM Tris-Cl (pH 7.4)

In addition, hypoxia, a hallmark of endometriosis, plays a key role in the mitochondrial function and metabolic conversion between glycolysis and oxidative phosphorylation and metabolic remodeling is known to contribute to cell survival and disease progression (36)

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Among the numerous cell types composing the epithelial layer are goblet cells which secrete glycosylated Mucin-2 (Muc2), a key component of the intestinal mucosal layer (Brenchley and Douek 2012

doi: 10.1007/BF02220605