glutathione s-transferase pull-down assays Pull down assay. Pull down assay was performed to study the interaction Detection of Protein–Protein Interactions Using

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Transient increase in human muscle sympathetic nerve activity during manual acupuncture

After centrifugation, 10 l of the supernatant were filtered through a Microcon YM-10 centrifugal filter device (Amicon), and analyzed by reversed-phase high-performance liquid chromatography on a C18 column (Atlantis 5 m, 4,6200 mm, Waters) as previously described [18]

at the University of Ljubljana (Slovenia), Ph.D