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and controlled delivery systems that achieve and bind to targeted site via different stimuli or due to specific conditions (temperature and pH), followed by antibacterial activity while providing controlled cell/ion/growth factor release from the matrix (Jacob et al., 2018) (Figure 3)

when possible, limit repeated freezethaw events to help maintain consistency across experiments

For example, fabricating gold-doped CDs requires specific control over reaction conditions, including reaction temperature, pH environment, and concentration of reactants to accomplish the anticipated size and functional attributes