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(88) established an innovative metastasis assay by microinjecting human breast cancer cells into duct of Cuvier, enabling real-time tracking of tumor cell invasion into the avascular caudal fin

doi: 10.3390/nu10070867

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An important aspect in these experiments is the selection from large libraries using recursive cycles of reassembly (optionally in combination with other directed evolution methods described herein) to maximally access all the fortuitous but complex mechanisms that cannot be approached rationally, such as DNA transfer into the cell

Costabile et al., 2017