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Samples were centrifuged at 16,000 g for 10 min at 4 C, and 200 l of supernatant was mixed with 200 l of 2,4-dinitrophenyl-hydrazine solution (0.05 M in acetonitrile:acetic acid 9:1 (v/v))

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A deeper understanding of HSP regulation and function in the heart offers promising opportunities for the development of targeted therapeutic strategies in cardiovascular diseases

In the quest for optimal health and wellness, various methods and treatments have emerged to deliver essential nutrients and medications efficiently

Acknowledgments The authors acknowledge kind sponsorship by Ferring Pharmaceuticals to support in vitro experimental work to determine the role of GH in carbohydrate and lipid metabolism in granulosa cell lines performed in the laboratory of KK and JY