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For example, silica cannot be used directly with sulfhydryl-reactive methods but must be reacted with aminopropyltriethoxysilane or mercaptopropyltrimethoxysilane to convert it into a suitable form.There are various approaches that can be used to immobilize ligands with sulfhydryl groups

196 Although the mutation frequency of E3 ubiquitin ligase is lower than that of other WNT inhibitors, mutations of RNF43 (which mediates ubiquitin degradation of Frizzled) are still detected in the adenoma tissues of patients with GC, 218 early-onset metastatic CRC, 219 and Lynch syndrome

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